FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377547
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6880.18222896751927575No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT6200.1642179649156264No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4870.12899056276437104No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG4360.11548231081163404No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4270.11309850164350399No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4120.10912548636328723No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3960.10488760339772267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAG350.001254709439.9999243
GCCTAGG459.183094E-538.8939671
CTCGTAT1650.033.9393339
GTCTTCT1600.030.62494349
GCCGTCT2000.029.74994346
GACATAT500.00719974128.0036561
TGCTTGA2250.027.99994955
TGACCGT500.00720439927.9999475
CGTACAC500.00720439927.9999473
ACCGTAC500.00720439927.9999478
AGGGATT500.00720439927.9999475
CCGTCTT2400.027.70828247
TCTCGTA2100.026.66661538
GTATGCC2250.026.44439542
TTAGACT808.655657E-526.249954
CTATACA808.655657E-526.249954
GTTCTAC700.001224096425.0032651
GTATTGG700.001224096425.0032651
TATGCAG700.001225042324.99995210
ATCTTTT4650.024.83866312