FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246698
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT13280.5383099984596551No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT6350.25739973570924773Illumina PCR Primer Index 10 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4510.18281461544074132No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3890.15768267274157066No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3780.15322377968204037No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3360.1361989152729248No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2970.12039011260731745Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT2740.11106697257375414Illumina PCR Primer Index 10 (95% over 24bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2730.11066161865925139Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT2600.10539201777071561No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGT150.002222827270.0000155
TGCTTAT352.082665E-550.012
TTAGCCG352.082665E-550.04
GTTAGCC352.082665E-550.03
TAGCCGG404.584265E-543.7500045
TGCTTGA3200.040.46875455
TCTTCTG3200.040.46875450
GTATGCC3200.040.46875442
TACTGCC350.001253852540.054
TATGCCG3250.039.84615343
GCCGTCT3300.039.24242446
GTCCTAA459.180895E-538.888891
GCTTATT459.180895E-538.8888913
AGTTATG459.180895E-538.888898
CCGTCTT3350.038.65671547
CTCGTAT3300.038.1818239
ATGCCGT3500.037.044
GTCTTCT3600.035.97222549
GCTTGAA3650.035.4794556
ATTTACT400.002415763235.00000451
Produced by FastQC (version 0.11.2)