FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250976
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5860.23348846104806836No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4770.19005801351523652No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG4710.18766734667856688No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4430.17651090144077522No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4300.17133112329465766No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4200.16734667856687493No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3950.15738556674741808No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3880.15459645543797015No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG3590.14304156572740023No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC3470.13826023205406093No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3360.13387734285349992No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3060.12192400867015171No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT3030.1207286752518169No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA3020.12033023077903864No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2950.11754111946959071No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2900.11554889710569936No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2810.11196289685069488No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC2770.11036911895958179No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA2690.10718156317735561No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2660.10598622975902079No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT2650.10558778528624249No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT2610.10399400739512941No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTAC252.383028E-456.020891
GTATATA252.383028E-456.020891
TGTTAGC200.00694644152.49865739
TCTTACA459.171961E-538.8956452
GTCTTAC1100.038.196061
TATAAGA654.843296E-737.6988533
GGTCCAA852.562956E-937.05787311
CATATAA708.63869E-735.0130541
CTTACAC806.208393E-835.006083
ACTTATG501.7069404E-434.99910410
TAGTTCT601.2129807E-534.999115
AAGAATT957.5961E-933.15704316
TACCGCC751.4836733E-632.672347
AATTAAG552.9872867E-431.8173750
TCACCTC1001.2523742E-831.49919323
TCCAAGA1001.2523742E-831.49919313
CAAGAAT1001.2523742E-831.49919315
ATAAGAA802.455141E-630.630324
TTCTAAC703.4807028E-529.99923118
AGTTCTA703.4807028E-529.99923116
Produced by FastQC (version 0.11.2)