Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s28.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 453026 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 1026 | 0.22647706754137734 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT | 999 | 0.22051714471134107 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 711 | 0.15694463452428778 | No Hit |
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT | 652 | 0.14392109945124562 | No Hit |
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA | 648 | 0.14303814792086988 | No Hit |
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG | 592 | 0.13067682649560952 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 591 | 0.1304560886130156 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 575 | 0.12692428249151264 | No Hit |
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT | 572 | 0.1262620688437308 | No Hit |
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC | 561 | 0.12383395213519754 | No Hit |
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA | 546 | 0.12052288389628851 | No Hit |
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT | 511 | 0.11279705800550077 | No Hit |
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA | 500 | 0.1103689412969675 | No Hit |
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC | 490 | 0.10816156247102815 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT | 485 | 0.10705787305805847 | Illumina PCR Primer Index 10 (95% over 22bp) |
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT | 483 | 0.1066163972928706 | No Hit |
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG | 465 | 0.10264311540617978 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 170 | 0.0 | 39.1174 | 39 |
TAAACTA | 45 | 9.1934126E-5 | 38.888645 | 5 |
GTATGCC | 200 | 0.0 | 34.99978 | 42 |
GCCGTCT | 215 | 0.0 | 32.55793 | 46 |
CGTATAC | 45 | 0.004305865 | 31.110914 | 26 |
TATATGG | 45 | 0.004305865 | 31.110914 | 2 |
TGCTTGA | 250 | 0.0 | 29.399815 | 55 |
TCTTCTG | 240 | 0.0 | 29.166483 | 50 |
ATATATA | 75 | 5.5641744E-5 | 28.012192 | 1 |
CGTATGC | 250 | 0.0 | 27.999825 | 41 |
GTCTTCT | 250 | 0.0 | 27.999825 | 49 |
TTAGTGA | 50 | 0.0072060507 | 27.999823 | 3 |
TATGCCG | 255 | 0.0 | 27.450808 | 43 |
ACTGAAT | 90 | 6.1650426E-6 | 27.22205 | 8 |
TCTCGTA | 245 | 0.0 | 27.142685 | 38 |
ATAAGAA | 155 | 4.3655746E-11 | 27.096603 | 4 |
GTTAGAG | 240 | 0.0 | 26.249834 | 62 |
AGTTAGA | 240 | 0.0 | 26.249834 | 61 |
TCTTTTT | 535 | 0.0 | 26.16806 | 8 |
CTTTTTT | 545 | 0.0 | 25.687912 | 9 |