FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453026
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10260.22647706754137734No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT9990.22051714471134107No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7110.15694463452428778No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6520.14392109945124562No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6480.14303814792086988No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5920.13067682649560952No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5910.1304560886130156No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5750.12692428249151264No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT5720.1262620688437308No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5610.12383395213519754No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5460.12052288389628851No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT5110.11279705800550077No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA5000.1103689412969675No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4900.10816156247102815No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT4850.10705787305805847Illumina PCR Primer Index 10 (95% over 22bp)
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4830.1066163972928706No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4650.10264311540617978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1700.039.117439
TAAACTA459.1934126E-538.8886455
GTATGCC2000.034.9997842
GCCGTCT2150.032.5579346
CGTATAC450.00430586531.11091426
TATATGG450.00430586531.1109142
TGCTTGA2500.029.39981555
TCTTCTG2400.029.16648350
ATATATA755.5641744E-528.0121921
CGTATGC2500.027.99982541
GTCTTCT2500.027.99982549
TTAGTGA500.007206050727.9998233
TATGCCG2550.027.45080843
ACTGAAT906.1650426E-627.222058
TCTCGTA2450.027.14268538
ATAAGAA1554.3655746E-1127.0966034
GTTAGAG2400.026.24983462
AGTTAGA2400.026.24983461
TCTTTTT5350.026.168068
CTTTTTT5450.025.6879129