FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402778
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT9690.2405791776114882No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG7290.18099300359006698No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6240.1549240524556952No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6120.15194474375462413No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6020.14946198650373158No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6020.14946198650373158No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5390.1338206158231085No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT5280.1310895828471267No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5080.12612406834534162No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4980.12364131109444906No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4770.11842752086757469No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4540.11271717919052182No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA4450.11048269766471853No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4410.10948959476436151No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4380.10874476758909375No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT4220.10477235598766567No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4140.10278615018695161No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC4120.10228959873677312No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA4060.10079994438623757No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA4050.10055166866114833No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGTA350.001253441440.00884
TGTGATA350.001254973139.99886343
TTAACTC501.7063066E-435.0076983
ACTAGTA552.9861723E-431.8251782
GTATGCC1357.2759576E-1231.11022842
AACTAGT604.9649796E-429.180331
TGTATCA604.9722055E-429.1730822
CTAGTAC604.9722055E-429.1730823
TATACAG604.9722055E-429.1730825
GTCTTCT1601.8189894E-1228.43669349
GTATTAA500.007188438428.0131191
GAGCATA500.007188438428.0131191
TAAGACA500.007197161728.0061594
CCCGTAA500.007205893327.99920750
AGTAAGA500.007205893327.99920747
ATATAAG1653.6379788E-1227.5818232
CCGTCTT1653.6379788E-1227.57497447
ATGCCGT1653.6379788E-1227.57497444
TATGCCG1554.3655746E-1127.09600443
ATATCTA657.9401E-426.9289995
Produced by FastQC (version 0.11.2)