FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559939
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT14840.265028869216111No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG11810.21091583190311802No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA10610.18948492603658612No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10120.18073397280775227No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA10050.17948383663220457No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT8850.1580529307656727No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC8790.1569813854723461No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC8490.15162365900571312No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG7710.1376935701924674No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT7650.1366220248991408No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA6800.12144179991034737No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT6670.11912011844147309No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC6590.11769139138370431No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA6460.11536970991483No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA6400.11429816462150341No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT6390.11411957373928233No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG6070.10840466550820714No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6030.10769030197932275No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT5870.10483284786378516No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5740.10251116639491088No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA5660.10108243933714207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG459.1962014E-538.8886383
GATGACT450.00430672831.110916
ATTCGTA604.9812515E-429.16648124
TATAGGA604.9812515E-429.1664814
GCTACGC1056.766604E-726.66649617
TATGCTT4500.026.4442755
AAGACTC808.663257E-526.2498324
ACCGGTA2700.025.92575815
ATAAGAA2900.025.3446664
TATAAGA3050.025.245743
TAAGTAG700.001225814524.9998425
ATAAGTC700.001225814524.99984255
ATGTCTA4200.024.16651233
CATATAA3050.024.1089731
TCTTTTT8200.023.90228713
GATGGTA4700.023.82963649
CTTTTTT8200.023.4754614
GTAATAA750.001827176123.3436071
CTTGTCT4500.023.3331859
GCATGTC4500.023.33318531
Produced by FastQC (version 0.11.2)