Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s25.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6659 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT | 2645 | 39.72067878059769 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT | 856 | 12.854783000450517 | Illumina PCR Primer Index 10 (95% over 22bp) |
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT | 373 | 5.601441657906593 | Illumina PCR Primer Index 10 (95% over 24bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 137 | 2.0573659708664964 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC | 119 | 1.7870551133803876 | No Hit |
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT | 90 | 1.351554287430545 | Illumina PCR Primer Index 10 (95% over 21bp) |
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG | 59 | 0.8860189217600241 | Illumina PCR Primer Index 10 (96% over 25bp) |
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG | 38 | 0.5706562546928968 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATATCGTATGCCGT | 30 | 0.45051809581018165 | No Hit |
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT | 27 | 0.40546628622916353 | Illumina PCR Primer Index 10 (96% over 27bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC | 15 | 0.22525904790509083 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATATCGTATGCCGTCTT | 12 | 0.18020723832407268 | No Hit |
GTACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT | 10 | 0.1501726986033939 | Illumina PCR Primer Index 10 (95% over 22bp) |
GCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG | 8 | 0.12013815888271512 | No Hit |
CTTAATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG | 7 | 0.10512088902237572 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTATAC | 295 | 0.0 | 59.322037 | 1 |
TTATACA | 305 | 0.0 | 57.37705 | 2 |
TATACAC | 315 | 0.0 | 55.555557 | 3 |
TACACAT | 405 | 0.0 | 43.209877 | 5 |
ATACACA | 405 | 0.0 | 43.209877 | 4 |
GAAAAAA | 430 | 0.0 | 40.697674 | 60 |
GCTTGAA | 430 | 0.0 | 40.697674 | 56 |
TTGAAAA | 430 | 0.0 | 40.697674 | 58 |
CTTGAAA | 430 | 0.0 | 40.697674 | 57 |
TGAAAAA | 430 | 0.0 | 40.697674 | 59 |
AGGGGGG | 390 | 0.0 | 40.384617 | 70 |
TGCCGTC | 425 | 0.0 | 40.35294 | 45 |
TTCTGCT | 425 | 0.0 | 40.35294 | 52 |
CCGTCTT | 425 | 0.0 | 40.35294 | 47 |
TCTGCTT | 425 | 0.0 | 40.35294 | 53 |
GCCGTCT | 425 | 0.0 | 40.35294 | 46 |
AGCCTAT | 435 | 0.0 | 40.229885 | 32 |
GTATGCC | 435 | 0.0 | 40.229885 | 42 |
ATCTCGT | 435 | 0.0 | 40.229885 | 37 |
CCTATCT | 435 | 0.0 | 40.229885 | 34 |