FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6659
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT264539.72067878059769No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT85612.854783000450517Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT3735.601441657906593Illumina PCR Primer Index 10 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1372.0573659708664964No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC1191.7870551133803876No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT901.351554287430545Illumina PCR Primer Index 10 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG590.8860189217600241Illumina PCR Primer Index 10 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG380.5706562546928968No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATATCGTATGCCGT300.45051809581018165No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT270.40546628622916353Illumina PCR Primer Index 10 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC150.22525904790509083No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATATCGTATGCCGTCTT120.18020723832407268No Hit
GTACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT100.1501726986033939Illumina PCR Primer Index 10 (95% over 22bp)
GCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG80.12013815888271512No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG70.10512088902237572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2950.059.3220371
TTATACA3050.057.377052
TATACAC3150.055.5555573
TACACAT4050.043.2098775
ATACACA4050.043.2098774
GAAAAAA4300.040.69767460
GCTTGAA4300.040.69767456
TTGAAAA4300.040.69767458
CTTGAAA4300.040.69767457
TGAAAAA4300.040.69767459
AGGGGGG3900.040.38461770
TGCCGTC4250.040.3529445
TTCTGCT4250.040.3529452
CCGTCTT4250.040.3529447
TCTGCTT4250.040.3529453
GCCGTCT4250.040.3529446
AGCCTAT4350.040.22988532
GTATGCC4350.040.22988542
ATCTCGT4350.040.22988537
CCTATCT4350.040.22988534