FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658389
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA47540.7220655266111675No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT46950.7131042590322743No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG45930.6976118981331705No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG44630.6778667322813716No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC35990.5466373223124931No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG31620.4802631878722154No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC31510.4785924430693709No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC28010.42543238116068166No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA27270.414192825214273No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA24910.3783477548986997No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG24700.37515815118417833No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC23180.35207149572669044No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG22860.3472111472093246No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA22610.3434139999301325No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA21730.3300480415073763No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA21430.32549146477234586No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG21110.3206311162549799No Hit
GTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGC21060.3198716867991416No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA21060.3198716867991416No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC20820.3162264254111172No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG20500.31136607689375123No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT19850.30149349396785186No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT19480.2958737159946475No Hit
GTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACC19320.2934435417359646No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA19100.29010205213027557No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG19040.2891907367832695No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT18960.287975649653928No Hit
ATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAG18300.2779511808368609No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT17880.27157197340781813No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG17620.2676229402374584No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG17390.2641295647406017No Hit
TAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATT16160.2454476001269766No Hit
AGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACA15760.23937216448026927No Hit
CAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACC15600.23694199022158635No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG15320.23268918526889118No Hit
CTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGAC14880.2260062060575131No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC14550.22099397164897955No Hit
GGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATT14450.2194751127373027No Hit
CTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGAACTTGGAATT13920.2114251605054155No Hit
TAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATG13800.20960252981140332No Hit
GTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCAT13310.2021601211441868No Hit
CGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCA12480.18955359217726905No Hit
GTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATC12020.18256684118355562No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC11990.18211118351005257No Hit
GCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGT11760.17861780801319585No Hit
GTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACT11680.17740272088385436No Hit
AGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTC11620.17649140553684828No Hit
GACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTGAT11380.17284614414882388No Hit
GTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCA11320.17193482880181776No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA11260.17102351345481165No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT11010.1672263661756196No Hit
TCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCC10900.16555562137277507No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT10670.16206224587591833No Hit
AGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATA10540.16008772929073845No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGA10520.1597839575084031No Hit
TTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACC10500.15948018572606773No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACA10170.15446795131753416No Hit
GTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACT10140.1540122936440311No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG10020.15218966295001893No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC9760.14824062977965913No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACG9420.1430765094799579No Hit
ACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTG9350.1420133082417841No Hit
GGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTG9220.14003879165660424No Hit
GAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTG9130.13867181863609507No Hit
CTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAA9030.13715295972441824No Hit
GAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATC8890.13502655724807067No Hit
ATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTT8770.13320392655405847No Hit
TCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGG8460.12849546392786027No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCAC8440.1281916921455249No Hit
GGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGT8300.12606528966917735No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT8250.12530586021333892No Hit
ACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGCG8100.12302757184582368No Hit
CCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGC8090.12287568595465599No Hit
GAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTT7850.11923042456663158No Hit
GAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTAC7820.11877476689312852No Hit
AACTGAAACTGTTCACATGAACTTGGAATTCCCAGTAAGTGTGAGTCATT7800.11847099511079318No Hit
GATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCAC7680.11664836441678096No Hit
GTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTC7670.11649647852561328No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA7580.11512950550510413No Hit
GTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAG7580.11512950550510413No Hit
CACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATC7480.11361064659342729No Hit
CAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCG7320.11118047233474435No Hit
GATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGG7300.110876700552409No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA7210.10950972753189983No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA7200.10935784164073216No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG6920.105105036688037No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6880.10449749312336629No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT6850.10404183544986323No Hit
GACCTGTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTA6830.10373806366752786No Hit
ATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACA6810.10343429188519249No Hit
GGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGT6800.10328240599402481No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6750.10252297653818639No Hit
CTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAAC6680.10145977530001261No Hit
GGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTAGACCG6650.10100411762650956No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT6630.10070034584417419No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA6630.10070034584417419No Hit
CCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATG6610.10039657406183881No Hit
TACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGC6610.10039657406183881No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTT200.00695143952.49863431
CTCGTAT1650.042.42313439
GTATGCC1650.040.3019842
TATGCAG350.00125553939.99895510
CGATCTG28000.037.257525
TCCGATC53750.036.3422163
CTTCCGA53850.036.3397371
CCGATCT53250.036.223274
TTCCGAT53900.036.1111832
CGAGTAA400.002418993534.9990926
AGGCCTA400.002418993534.9990952
ACAGAGT400.002418993534.9990921
ATCTGGG10750.034.8442427
ATCTGTG2750.034.370577
TATGCCG1950.034.10167743
GATCTGG16050.033.8074466
TACAGGA1253.6379788E-1233.6067852
ATGCCGT2100.033.33246644
GCCGTCT2000.033.24913446
ATCTAAC1305.456968E-1232.3142177
Produced by FastQC (version 0.11.2)