FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211164
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6350.30071413687939236No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC6010.28461290750317286No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT5530.26188176014851017No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4980.2358356538046258No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4620.21878729328862873No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC3320.15722376920308387Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2850.13496618741830996Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT2520.11933852361197932TruSeq Adapter, Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGG200.006926949652.5330541
TGCCTTA200.0069464552.49573556
CGATAAA200.0069464552.49573557
CTAGCTC200.0069464552.4957359
AAGACTA503.4523728E-642.026455
TTCGGAA701.9048457E-840.0251852
TGTCTAG350.001253942839.99675429
GTCTAGG400.002407529935.0220381
AAGGAGC601.20663935E-535.0220386
GGTTGCA806.2143954E-834.99715816
GTCTTCT1102.3646862E-1134.99715849
GGTAACG957.588824E-933.155226
TTTCGGA851.0521762E-732.9619181
CAGGGGT552.974236E-431.8382154
GGGTAGG450.00428734431.1307011
TCGTAAG450.00428734431.1307011
CAACGGG901.748449E-731.10858521
AACGGGT901.748449E-731.10858522
AGAAACG901.748449E-731.10858567
GCTTGAA1251.03682396E-1030.79749956
Produced by FastQC (version 0.11.2)