FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501031
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11030.22014605882669935No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA7180.14330450610840448No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6640.13252672988298128No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT6430.1283353724619834No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5880.11735800778794125No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5700.11376541571280022No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT5650.11276747346970546No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG5470.1091748813945644No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5440.10857611604870757No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA5350.10677982001113705No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT5330.10638064311389914TruSeq Adapter, Index 12 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5170.10318722793599597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGT556.6837238E-638.1884084
CTCGTAT2900.036.20591439
ATAGGTA501.7073765E-435.006045
GTCTACG552.9827905E-431.8332041
CCGTCTT3450.031.44842547
TATAGTG450.004302530531.116485
CGATTAA450.00430673631.11026615
GTATGCC3500.030.9991642
GCCGTCT3550.030.56255146
CGTATGC3500.029.99918741
TCTCGTA3600.029.16587438
GACGTTA500.00720750227.99924329
CGTCTTC3900.027.8197648
GCTTGAA3950.027.4676156
ATGCCGT4000.027.12426644
GTCTTCT3900.026.92234849
ATTGTAC808.650933E-526.254533
TGTTACA808.650933E-526.254532
TGCTTGA4000.026.2492955
TATGCCG4150.026.1438743
Produced by FastQC (version 0.11.2)