FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757190
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC36950.4879884837359183No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC33170.43806706374886095No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC32060.4234075991494869No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC27170.3588267145630555No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT14240.18806376206764486No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC13460.17776251667349013Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA13060.1724798267277698Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG12710.16785747302526446No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA11280.14897185646931418No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10660.1407836870534476No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA10600.13999128356158957No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT9820.12969003816743485No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC9800.12942590367014886No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC9660.12757696218914671No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC9190.12136980150292528No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG8830.11661538055177698No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT8120.10723860589812334No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA7640.10089937796325889No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAT601.2155273E-535.0019843
GAACGTA703.489512E-529.99971618
ACTGGGT1103.180685E-828.6379878
TTAAGGT755.5834338E-528.0015854
GTCCTGA657.948048E-426.9281561
GGTCCAA3700.026.48623711
GCCGTCT1900.025.78922846
TGTACGA700.001225763725.0014152
GTCTTAC4950.024.7521461
CGACGGT4200.024.1680347
CAAGAAT4150.023.61423515
TATGCTT4450.023.5968425
AATAATC901.9272872E-423.3346547
AACGTAT750.001832556423.33311319
GTATGCC2103.6379788E-1223.33311342
ACGTATT750.001832556423.33311320
CGACTCA750.001832556423.33311359
GAATCAG4850.023.09256635
CGGTCCA4250.023.05860510
ACGAGAG2004.3655746E-1122.74978469
Produced by FastQC (version 0.11.2)