FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214359
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT6020.28083728698118576No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT3190.1488157716727546Illumina PCR Primer Index 10 (95% over 21bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT2750.12828945833858155No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT404.5822453E-543.7519
ATAATAC350.001253485340.03
TTCTACA459.1768685E-538.888892
CTCCGTG400.002415059635.032
GGAACGT708.6560976E-735.017
TATACAG400.002415059635.05
GGGAACG1055.456968E-1033.33333616
CGGGAAC851.0581425E-732.94117715
AGCGTTA751.4843481E-632.66666820
GAACGTA652.0960942E-532.30769318
AAGCGTT552.985395E-431.8181819
TTCCGAT21600.031.5972212
CTTCCGA22050.031.111111
CGATCTG10400.030.9615385
TCCGATC22050.030.952383
CCGATCT22150.030.6546294
CGATCTT4150.030.3614465
GATCTTA703.477749E-530.06
CGTATTC604.970938E-429.16666621
TACCCTA500.00719749527.9999985
Produced by FastQC (version 0.11.2)