FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818627
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC24790.3028241189210715No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC23640.2887762069904853No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC22950.28034745983213355No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC17860.21817018006979977No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC12660.1546491869923665Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA11490.14035696354994398Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACA751.4870802E-632.6780741
ATACAAT802.4654455E-630.6282063
GTACATG952.814304E-729.4839741
GCCGTCT3750.028.93282946
TAATATC853.9577026E-628.82302769
GTATGCC3750.027.99951242
GCTTGAA3700.027.43195356
TGCTTGA3850.027.27225155
CATACAA906.167689E-627.2250732
TTTCGGA2150.026.0556051
GTCTTCT4100.025.6093149
CTAGACA1659.640644E-1125.457214
TCTTTTT12200.025.2454613
TTCACTA1251.0982876E-725.2026398
CTTTTTT12300.025.04021314
CCGTCTT4350.024.94209547
CAAACAT851.3076403E-424.708475
CTAACAT851.3076403E-424.708473
CATACGT1001.3969895E-524.5025652
GGGTATG1301.6010381E-724.2392311
Produced by FastQC (version 0.11.2)