FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735673
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC14110.19179717075385397No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC12750.17331069646432587No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC12360.16800942810188765No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAT12260.1666501285217753No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC10420.1416390162477079No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC9800.13321135885101126Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA9080.12342440187420227Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC1550.036.128893
CCTTACA1004.4211447E-727.9998932
TGCGTAA500.00720884427.99989310
TCTACTT1705.456968E-1226.76462
CGAGAGT700.001226162724.99990370
TTTCGGA1902.1827873E-1123.9537871
GGGCATA901.9280051E-423.3332426
CTTTTTT7850.022.73876614
TTACACT1102.9217015E-522.272644
TCTTTTT8100.022.03695113
GCTATCG1755.2459654E-921.99991413
ATGTCGT800.002666304121.87491469
TAACCGA800.002666304121.87491438
CTCTACT2108.367351E-1121.6724741
TTAGGAC3100.021.4515293
CTACTTG1807.2595867E-921.3888053
ACACAGA1807.2595867E-921.3888056
CCTACAC1807.2595867E-921.3888053
GTATAAT1154.1084175E-521.3100591
GGGATAT1154.117041E-521.30426616
Produced by FastQC (version 0.11.2)