FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62174
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT26124.201113005436357No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT7361.1837745681474572TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT2970.47769163959211247RNA PCR Primer, Index 12 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1360.21874095280985623No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT1200.19300672306752018No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG1040.16727249332518418No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC1010.16244732524849614No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG1010.16244732524849614No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG1000.16083893588960013No Hit
ATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTT930.14958021037732816No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA910.14636343165953614No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC900.14475504230064012No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT850.13671309550616012No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT820.13188792742947214RNA PCR Primer, Index 12 (95% over 21bp)
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG780.12545436999388812No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA710.11419564448161611No Hit
ATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTT680.10937047640492811No Hit
GGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAA680.10937047640492811No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC680.10937047640492811No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC660.1061536976871361No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCCA150.002214988270.035
GGGTCCA150.002214988270.025
GGGTAGA150.002214988270.06
ATGCCAC150.002214988270.036
TTAGGAT200.0069212952.554
TCACCCC200.0069212952.513
TCGGGTC200.0069212952.523
TATATAG200.0069212952.55
CCCCGCT200.0069212952.516
GTCGTCC200.0069212952.550
CGGTGAT200.0069212952.547
ACAGAAG200.0069212952.536
ACCAAGG200.0069212952.530
AGTCGTC200.0069212952.549
CACTTGA200.0069212952.540
CTTAGGA352.0645486E-550.053
GTAATCT4800.045.20833234
GCCGTCT4950.044.54545246
CTGCTTG5050.044.35643454
TGCTTGA5050.044.35643455
Produced by FastQC (version 0.11.2)