FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126661
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT9920.783192932315393No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT7090.559761884084288TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT3220.2542218994007627RNA PCR Primer, Index 11 (96% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1940.15316474684393774No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA1650.13026898571778212No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1640.1294794767134319No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1600.12632144069603113No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1380.10895224260032686No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1350.10658371558727626No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1350.10658371558727626No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1340.10579420658292607No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1330.10500469757857589No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1290.1018466615611751No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCA150.00222067169.997248
GACGTAA207.902998E-569.997238
CGTAATC207.902998E-569.9972310
AGTTAAT207.902998E-569.9972323
GACTCAC207.902998E-569.9972330
CGAGTTA252.3833594E-455.9977921
GTTAATG252.3833594E-455.9977924
ACGTAAT252.3833594E-455.997799
ACTCACA252.3833594E-455.9977931
TAATGAC252.3833594E-455.9977926
AATACGC452.6788257E-854.44229521
TATGAGA200.006928085352.5186584
CTGACAC200.006938918552.4979259
GAGGTTC200.006938918552.49792566
AGGTTCG200.006938918552.49792567
ACCAGCC200.006938918552.49792569
CGTAAAC200.006938918552.49792527
TTCGAAG200.006938918552.49792570
TCACACT305.8606395E-446.6648333
CTGACTG305.8606395E-446.664839
Produced by FastQC (version 0.11.2)