FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692991
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT23620.3408413673482051No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC19310.2786471974383506No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC17880.25801200881396724No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC16690.2408400686300399No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC12100.17460544220632013No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC11380.16421569688495233Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA10230.1476209647744343Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA252.387938E-456.0115471
GTATGCC4500.035.7774142
GTCTATA400.002416326735.0072171
CTCGTAT4500.034.9996439
GTCGTAT400.00241889434.9996448
GTCTTCT4950.032.52491849
CCGTCTT5100.032.2545747
TATGCCG5000.032.19966543
TGCTTGA5100.031.56830255
GCCGTCT5250.030.6663546
GCTTGAA5300.030.37704756
CGTATGC5500.029.27242741
CTTGAAA5650.029.11474457
ATGCCGT5650.028.49528144
TCTTCTG5700.028.24532350
TTGAAAA5850.028.11936858
TCGTATG5750.027.99971240
TATTGGG1403.947207E-1027.50172
TCTCGTA5750.027.39102438
TAGGATG657.953254E-426.9247425