Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s15.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 692991 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT | 2362 | 0.3408413673482051 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 1931 | 0.2786471974383506 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 1788 | 0.25801200881396724 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 1669 | 0.2408400686300399 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 1210 | 0.17460544220632013 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 1138 | 0.16421569688495233 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 1023 | 0.1476209647744343 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATTGA | 25 | 2.387938E-4 | 56.011547 | 1 |
GTATGCC | 450 | 0.0 | 35.77741 | 42 |
GTCTATA | 40 | 0.0024163267 | 35.007217 | 1 |
CTCGTAT | 450 | 0.0 | 34.99964 | 39 |
GTCGTAT | 40 | 0.002418894 | 34.99964 | 48 |
GTCTTCT | 495 | 0.0 | 32.524918 | 49 |
CCGTCTT | 510 | 0.0 | 32.25457 | 47 |
TATGCCG | 500 | 0.0 | 32.199665 | 43 |
TGCTTGA | 510 | 0.0 | 31.568302 | 55 |
GCCGTCT | 525 | 0.0 | 30.66635 | 46 |
GCTTGAA | 530 | 0.0 | 30.377047 | 56 |
CGTATGC | 550 | 0.0 | 29.272427 | 41 |
CTTGAAA | 565 | 0.0 | 29.114744 | 57 |
ATGCCGT | 565 | 0.0 | 28.495281 | 44 |
TCTTCTG | 570 | 0.0 | 28.245323 | 50 |
TTGAAAA | 585 | 0.0 | 28.119368 | 58 |
TCGTATG | 575 | 0.0 | 27.999712 | 40 |
TATTGGG | 140 | 3.947207E-10 | 27.5017 | 2 |
TCTCGTA | 575 | 0.0 | 27.391024 | 38 |
TAGGATG | 65 | 7.953254E-4 | 26.924742 | 5 |