FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958633
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAC22480.23450058572988827No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA14750.1538649305834454No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT14380.15000526791796234No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC13800.14395498590179973No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC13000.13560976932778238No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC12530.13070695459054715No Hit
GTATTTAGCTTTAGATGAAATTTACCACCCATTTAGAGCTGCATTCCCAA11720.12225742280935457No Hit
GTCTAAGTGCCCTGGAACGGGCCATCGGAGAGGGTGAGAATCCCGTCTGG11600.12100564032325195No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACACGGGCACGGACACC10710.11172158688465762No Hit
GGCACGGACACCCCATCCCAGACGGGATTCTCACCCTCTCCGATGGCCCG10660.11120001084878155No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAA604.9712375E-429.1791271
TTACACT1103.1743184E-828.6456034
GTATAAA755.5708802E-528.011961
GTTAGTA755.5887333E-527.9988119
TACATTG1900.025.7977985
ATACAGT700.001224100825.008076
GTATGCC3700.024.5935542
GCCGTCT3700.024.5935546
CGTTATT3200.024.06273310
CTCGTAT3800.023.94635239
CTTTTTT6300.023.889129
CTTATAG3150.023.34331
TCTTTTT6600.023.3396478
ACCGTAC4350.022.534838
GGGTATT4200.022.49904416
TATACCG1253.0824904E-622.407235
CTACATT2350.022.3476354
ATATAAG1102.9155319E-522.2799152
GACCGTG1102.916707E-522.2787517
AACGTAT1102.9237663E-522.2717819
Produced by FastQC (version 0.11.2)