FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102360
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGACCTGTCGGC30870.2800355600711201No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC24390.22125258536231357No Hit
GGTGTGCACCGGTCGTCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCT23640.21444900032657208No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT23360.21190899524656193No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC23210.2105482782394136No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC21730.19712253710221705No Hit
GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTT19480.17671178199499255No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGG19300.1750789215864146No Hit
ACACTGATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAA19080.17308320330926377No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCC18460.1674589063463841No Hit
CCCTAACGTTGCCGTCAACCGCCGCGTCCCGGTTCAGGAATTTTAACCCG17980.16310461192350956No Hit
ACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGG16540.15004172865488588No Hit
GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATT16050.1455967197648681No Hit
GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTATAGCC15270.13852099132769694No Hit
CCTATAAGCAACATCCGCCGATCCCTGGTCGGCATCGTTTATGGTTGAGA14400.1306288326862368No Hit
GATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAATCTTT14170.12854239994194275No Hit
CTTCCGATCTGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTT13830.12545810805907326No Hit
CTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGT13630.12364381871620886No Hit
TCATTACTCCGATCCCGAAGGCCAACGTAATAGGACCGAAATCCTATAAT12960.11756594941761311No Hit
CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC12880.11684023368046736No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT12530.11366522733045467No Hit
ACTGATGACAGCGTCGCAATAGTAATTCAACCTAGTACGAGAGGAACCGT12430.11275808265902246No Hit
ACGTAATAGGACCGAAATCCTATAATGTTATCCCATGCTAATGTATCCAG12190.11058093544758518No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC11910.10804093036757502No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT11810.10713378569614282No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGG11770.10677092782756994No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC11520.10450306614898945No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC11400.1034144925432708No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11370.10314234914184114No Hit
CCTATAATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA11370.10314234914184114No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT200.00695215852.49962628
GTCTAGG901.05501385E-1038.895671
TAATGTG556.697199E-638.1832775
CTGTACG501.7105097E-434.9997529
CATAAGC400.00241947534.99975259
TACACCG1154.0017767E-1133.479545
GTATTGG957.6306605E-933.1636771
CCTGGGT1950.032.3089263
GGTCTAC552.9935027E-431.81795551
TATGGCG450.004307579731.1122995
CTAGCGG450.00430853631.110899
ATCTGGG12050.030.2086227
AGATACT703.4898057E-530.0011466
TCTACTC703.4898057E-530.0011468
TCCTGGG3900.029.6165162
CTAAAGT604.983045E-429.1677844
TATACTC604.983045E-429.1677843
GTTAGTG1202.3028406E-929.1664660
GGGCCCT2300.028.914156
CGTATGC853.959023E-628.82332241
Produced by FastQC (version 0.11.2)