FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944270
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT27370.2898535376534254No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC21830.23118387749266628No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT20530.21741662871848094No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC19540.20693233926737056No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC19240.2037552818579432No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT14610.15472269583911383TruSeq Adapter, Index 11 (95% over 23bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC14150.14985120781132516No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA13310.14095544706492846No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA13210.13989642792845267No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG12780.13534264564160675No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT12560.13301280354136No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT12300.13025935378652292No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC11940.12644688489521005Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA11300.11966916242176495Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC11120.11776292797610853No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC11090.11744522223516578No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA10470.11087930358901585No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG10100.1069609327840554No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT9880.10463109068380866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA708.6888394E-735.004587
GTATGCC6300.028.88808342
GGTCCAA3700.028.37758411
GTTATGT500.00721071427.99921636
TGCTTGA6500.027.46077255
CTCGTAT6650.027.36765539
GCTTGAA6550.027.25114656
GTCTACT657.932531E-426.9380131
GTCTTCT6650.026.84135249
ACTTTAG1207.367271E-826.2645651
TATAATA808.65823E-526.2548272
GCCGTCT7200.026.24926646
CCGTCTT6950.026.18631747
GAATCAG8250.024.6053735
TATGCCG7450.024.42884443
CGTAATT1151.4977195E-624.34714517
TATGCTT4900.024.2901785
TCTTCTG7400.024.12094750
TGAGAAA7150.023.98534265
CTTCTGC7600.023.94669751
Produced by FastQC (version 0.11.2)