FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674187
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA11500.17057581946848577No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT11370.16864757107449416No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC9080.13468073398033484No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG8980.1331974659849567No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC8430.12503949201037695No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC8180.1213313220219316No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC7720.11450828924319217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACATA350.001254595140.005216
CTCGTAT3450.031.4487139
GTATGCC3750.028.93281442
TCGTATG3800.028.55211840
GCCGTCT4000.027.1245146
ATCTTTT11050.026.924597
GTACTAT1606.548362E-1126.2573171
CTAGACA1207.390918E-826.2534224
GATCTTT12800.026.2534226
TTATACC808.6621454E-526.2514723
TCTTTTT11100.026.172648
CCGTCTT4400.025.45408647
TCTTCTG4700.024.57402650
TCTCGTA4600.024.34738738
CTTTTTT11800.024.3233999
ATCTGGG9550.023.8233287
TGCCGTC4850.023.81400545
GTCTTCT4750.023.57852449
GTATATG1652.6484486E-923.3398381
TATGCCG4750.022.84169443