FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_neb8g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_neb8g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6849831
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG66800.047.970966145
CACGTCG107400.036.85709145
AACTCCC104500.033.994797145
CTCCAGG128650.031.276333145
CACACGG148000.029.342543145
CACCCGG135850.027.217186145
CGGAAGG78800.025.669102145
CGTCTGG48850.024.636341145
AAGAGCC203150.023.803547145
ACACGTG41150.021.318085145
ATCGGAG79450.021.170286145
GAGCACC259150.020.422285145
TAACGTA62450.020.204044
ACGTAAT66950.020.0374576
CCAGTCC206200.019.759699145
GTCTGAC135850.019.745802145
AACGTAA70350.019.0690525
TTAACGT68300.018.6858753
CGTAATT72800.018.4235367
CCGTCCC152700.017.424475145
Produced by FastQC (version 0.11.2)