FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_neb7g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_neb7g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8510245
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA136390.1602656562766407No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT130970.15389686195873328No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC117930.13857415385808516No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG101680.11947952144738488No Hit
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCA99010.11634212646051906No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG92250.10839875937766774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG90850.048.599102145
GTCACCC99800.039.664135145
CACGTCG136950.039.598198145
AACTCCC124050.038.982075145
CTCCAGG166850.034.196716145
CACACGG179950.033.278522145
CGGAAGG89500.028.432823145
CGTCTGG63550.027.037634145
AAGAGCC257600.022.881294145
TAACGTA90550.022.342714
GTCTGAC164700.022.185684145
GACTCTA45300.022.0902674
GCTGACT43200.021.9915641
AACGTAA99200.021.7102535
ACACGTG42900.021.631594145
ACGTAAT98600.021.4746486
TTAACGT97350.020.8564133
CGTAATT101500.020.428597
ATCGGAG96850.019.912138145
ACCAGAG151400.019.633326145
Produced by FastQC (version 0.11.2)