FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_neb37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_neb37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7748234
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG56150.047.51513145
CACGTCG72550.041.57093145
CACACGG103700.037.333294145
GATCGGG49100.037.06176145
CCAGTCC91550.030.963657145
AACTCCC79000.029.091492145
CGGAAGG62150.028.113165145
GAGCACC164000.026.833584145
CTCCAGG97950.026.794025145
AAGAGCC194550.023.551561145
CGATGTG47700.021.278622145
GTCTGAC100550.019.900356145
CGTCTGG49000.016.867186145
CGTCTGC122350.016.591585145
ACACGTG44950.016.290167145
CGGAAGC202550.015.713253145
GTCACCC48000.015.708182145
CTGACCG36050.015.28418145
CCGATGG66600.015.240093145
CACCGAG113600.014.806196145
Produced by FastQC (version 0.11.2)