FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_neb142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_neb142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8647673
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG62950.046.87395145
CACGTCG76900.040.91629145
GATCGGG53300.040.262188145
CACACGG109150.040.185047145
AGTCACG54500.037.77937145
CCAGTCC87000.032.249622145
GAGCACC166900.030.624264145
CGGAAGG67700.029.449429145
AACTCCC88000.028.505344145
CTCCAGG100000.027.477175145
AAGAGCC207650.025.487299145
CTGACCC65000.020.968983145
GTCTGAC98500.020.8297145
CGTCTGG54050.017.03495145
CGGAAGC205600.016.643772145
CGTCTGC129700.016.377987145
ATCGGAG101350.016.095024145
ACACGTG52450.016.034128145
TCACTGC117150.015.904635145
GCCGAAT26500.013.9575071
Produced by FastQC (version 0.11.2)