FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_lex8g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_lex8g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335939
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA452670.71444816624655No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG306590.48389039099019104No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC282770.44629533207311495No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA263510.4158973121426832No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA236610.37344109531357544No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG235360.37146822278434183No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA199320.31458636202147783No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT180640.28510375494461043No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA155080.24476245746684117No Hit
CGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATG108430.17113485467584205No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT106760.16849909697678592No Hit
CTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTT106530.16813608843140693No Hit
TCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACG105340.16625791378357652No Hit
TCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGG100640.15883991307365808No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA99220.15659872988044865No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT95020.14996987818222365No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA91830.14493510748761945No Hit
TCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAG78300.12358073523119462No Hit
TCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAA77010.12154473078102551No Hit
CCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGT76600.12089762859143688No Hit
TTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATA74710.11791464532723564No Hit
CAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGAT66640.10517778027850332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCC14350.062.669331
TGCGACC32200.059.010691
GCGTCCC8000.058.019671
TACCTGG5100.056.8820271
GCGACCC146250.055.98651
AGGTCAG384200.048.4221469
CAGGTCA379100.048.0982138
CCAGGTC350550.047.8786967
CCCAGGT317000.047.5132266
CGATCCC21500.046.2075082
CGACCCC201650.044.9512942
CCCCAGG304400.041.856645
GACCCCA240500.041.0666243
ACCCCAG251050.040.872394
TCCAGGT20300.040.723596
TCGACCC16250.039.716142
CGTCCCC11450.038.632672
TTGCCGT10400.036.9596441
GCGCGAT16700.035.6108741
CGTACCC7900.034.880842
Produced by FastQC (version 0.11.2)