FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_lex7g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_lex7g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8539623
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG424260.49681350101755073No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC356020.41690365019626746No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA317320.37158549036649513No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA286850.3359047583248113No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA208580.24424965832800816No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT205810.24100595541512781No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA192460.2253729467916792No Hit
TCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACG140620.16466769083365856No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT137780.1613420170890448No Hit
CTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTT131270.153718729737835No Hit
CGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATG102000.11944321195443874No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT101270.11858837328064717No Hit
CCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGT98050.114817715020909No Hit
TTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATA94610.11078943414715146No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA91150.10673773303575579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTCA61600.039.310028
CCCAGGT54300.039.126776
CCAGGTC60850.037.7690667
CGCGATG20950.036.3417972
AGGTCAG67650.036.3303389
GCGACCC32650.031.319761
TTGTCGG55450.029.2973941
CCCCAGG66200.027.3834555
ACCCCAG52750.025.1554624
TGTCGGT61900.025.0682622
GACGGTC56900.024.0853633
GCGGTCT51950.023.7283254
TGCGACC9850.023.5611381
TTGCCGT23250.022.7710171
GGCTTGC49850.022.6916286
CCCGATT9000.022.5630631
GTCGGTC64400.022.1811493
GCTTGCG53000.022.1638586
ATTGCCG23650.022.0792261
TGTCCGG29750.021.9400711
Produced by FastQC (version 0.11.2)