FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_lex37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_lex37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7024189
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCC25150.028.548611
CCCAGGT42650.028.0529466
AGGTCAG51050.026.8410349
CAGGTCA49650.026.1376768
CCAGGTC50250.024.8156437
CCCCAGG51850.020.697915
ACCCCAG49500.019.6296774
GACCCCA53450.017.7720343
CGACCCC49800.016.5993922
GGTCAGG54600.016.3322359
GTCAGGC45950.016.03028910-14
TACCCGC39800.015.11917720-24
CCGCTGA48750.014.6931725-29
ATTACCC50000.014.41283920-24
TCAGGCG51750.014.09356310-14
GCGGGAT53550.013.67398415-19
TTACCCG51400.013.39965620-24
CCCGCTG44900.013.2726825-29
GCGCCTT17500.013.2594496
GGCGCCT14300.013.1841125
Produced by FastQC (version 0.11.2)