FastQCFastQC Report
Wed 20 Jun 2018
H3VLHAFXY_n01_lex142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VLHAFXY_n01_lex142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90875903
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGT468650.033.328546
AGGTCAG536800.032.603229
CAGGTCA546350.031.7547158
CCAGGTC528550.031.4250327
GCGACCC321000.029.823021
CCCCAGG582600.024.8557175
CGACCCC474750.023.063832
ACCCCAG558200.022.6944984
GTCAGGC495250.017.64577910-14
CCGCTGA488000.017.19486625-29
TACCCGC427300.016.84128220-24
GCGGGAT527350.016.32142315-19
ATTACCC532600.016.24223920-24
TTACCCG500900.016.03697820-24
TCAGGCG544500.015.82336610-14
CGCTGAG508550.015.76727425-29
CCCGCTG464950.015.63351325-29
GGTCAGG552350.015.3701579
GAGCGGG295700.014.171323145
TGCCCCA252850.014.1644827
Produced by FastQC (version 0.11.2)