FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509868
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGA1351.0195436E-842.9857034
TTAATAG1401.4055331E-841.4504973
CACTATA700.001053407641.4504971
TACACGG1354.944468E-737.6124885
GTGTTAA1406.573864E-736.2691841
TTATTAC800.002030436.2691843
TATATGG1051.8477585E-434.5386922
TTAGGAC1905.3878466E-934.360283
TATAGGT2206.184564E-1032.9719853
TAGGACC2206.184564E-1032.9719854
TTATACC2653.6379788E-1232.83145145
CTAACGT1352.1324244E-532.2392773
GTAGCAC1352.1324244E-532.2392773
ACCTATA2859.094947E-1230.542471
GACTAGG1204.0427316E-430.224321
CTTAATA1204.045057E-430.2213522
TAACGTT1453.474501E-530.0158754
AGGACTA1702.9955208E-629.868745
CCTTTAA1255.1344134E-429.0153471
GTATAGA1255.1344134E-429.0153471
Produced by FastQC (version 0.11.2)