FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743891
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10120.13604143617814976No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9400.12636259882160156No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7870.10579506943893661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG4900.035.5091679
CTATAAG6250.034.8013232
CCTATAA6250.033.641281
TATAGGT4750.033.582483
GTATAGA2653.6379788E-1232.8314361
CTTCCGA11000.032.296681
TTCCGAT11100.032.0057222
TATTAGG1153.1606219E-431.5229362
TATAAGC7850.029.557253
CCGATCT11300.028.874724
CCCCCCG3301.8189894E-1228.5597749
AAGGTAG3850.028.2497276
TCCCTAG1306.479936E-427.8856751
AGGTCGC6350.027.4044596
ACCTATA4850.026.908241
GTCGCGC6300.026.4674828
TAGGCGT1100.009665064526.3664114
ATACCCG4950.024.901615
TACCCGG5250.024.8597586
TAGGTCG6850.024.3456295
Produced by FastQC (version 0.11.2)