FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458459
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGTA803.7253216E-545.325155
TAGACTC700.001054592341.4401365
GTAGGAT1157.1206014E-637.8366433
CTATAGT1001.3879933E-436.260121
CCCTAAC1406.5848144E-736.2601171
GACTAGG1956.9558155E-933.470881
CTAGGAC2206.184564E-1032.9637453
TGTTAAG2206.202754E-1032.9601482
CTAGTCT1551.459528E-632.7510764
TCTAGTC1153.15428E-431.5305373
TAGGATA1504.3852182E-529.0080954
ACTAGGA2752.1282176E-1029.0049322
TGTAGGA1504.388483E-529.004932
AGTATAC1000.00606926629.0017688
TAAAGGT1555.483577E-528.0723514
TATATAG1050.007693681427.6267573
TCTATTC1050.007693681427.6267573
TACTGTG1606.8173744E-527.1891577
GTTAAGA1907.1289014E-626.717983
GTGTAGG2456.4050255E-826.6400871
Produced by FastQC (version 0.11.2)