FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956106
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18010.18836823532118824No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC17610.18418459877879648No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16140.16880973448550682No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15070.15761850673460892No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC14780.15458537024137492No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT14720.15395782476001615No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG13340.13952427868876463No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12560.13136618743110073No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11880.12425400530903477No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC11280.11797855049544714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC7650.045.4915738
TCGCGCG7750.044.9045879
TATAGGT8000.044.4145663
GGTCGCG8000.044.4075937
AGGTCGC8650.039.4004366
TATACTG2700.034.9139445
GTATAAG2103.783498E-1034.5284651
CCTATAA9200.030.7378431
TAGGCGG4800.030.2139914
TTCCGAT8650.030.1759012
CTATAAG9900.029.2953512
TCAGGGG1804.692838E-628.1997243
CTAGGCT5450.027.9410114
CGCCTAG5850.027.2686371
ATACCCG6650.027.2607425
GTATACG1606.821559E-527.1911681
CCTACAC5250.026.2430083
TAGGTCG13700.025.9355135
GCGGATC5650.025.6644947
CTAGGCG6550.025.4627823
Produced by FastQC (version 0.11.2)