FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610226
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG16670.2731774785079626No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10790.17681973563892722No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC9450.15486065818237832No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9410.1542051633329291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGT502.0050255E-458.0268634
ACCTATA6550.044.295311
ATACCCG6250.044.100415
GTGTTAA2650.038.3196261
TATAGGT4550.036.665323
GTTAAGA2850.035.6305273
GAATATA1458.65959E-735.016211
TTAAGAA3150.034.53984
GAATTAG850.002734934434.133451
TACCCGG7700.033.91186
ACACATA4550.033.463318145
TAAGAAG3100.032.75715
GGTATAA900.003618915632.2371441
CTATACC8350.032.1406253
CCTATAC9500.030.5379492
CTAACGT8600.030.3628943
TAACGTT9950.029.1592274
TATACCC9250.029.0134324
GTATAGA1255.137569E-429.013431
CCCTAAC9050.028.8531341
Produced by FastQC (version 0.11.2)