FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511164
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9450.18487217409676737No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8770.1715692028390106No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG8110.15865749544177601No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5740.11229272796988833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCC1850.047.044043
TATAGGT4550.039.8495753
CCTATAA2250.038.684441
ATACCCG4800.037.774085
TAGACGT1655.987931E-835.1642344
TAAGAAG3000.033.8455735
CTATAAG2404.3655746E-1133.2379342
ACCTATA5100.032.711111
TGTTAAG3150.032.2307242
GCTAGGG900.003621901232.2307242
TACCCGG5700.031.809756
CTATTCA1601.8694573E-631.7302234
TAGACAG3250.031.2420675
GTGTTAA3050.030.9158461
GTTAAGA3300.030.7687033
GTAGTAC950.00471680230.537363
AGGTCGC6250.030.1709126
CTAGACG1453.4784898E-530.0108533
CTAACGT5150.029.5738013
GTGTATT1000.006058096429.013331
Produced by FastQC (version 0.11.2)