FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980692
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAG1656.0052116E-835.159075
GTGTTAA4250.032.4203831
TAGGACA1352.1390135E-532.229154
TATACGG1453.48521E-530.0064475
CGCTATA1504.392178E-529.0062332
TACACTG2553.0158844E-928.4374835
TAATGGT2354.2884494E-827.7719254
CCATACT2104.976919E-727.6249852
TTACACT2903.947207E-1027.5059094
TATAGGT5100.027.0156083
TTATACC4600.026.796505145
TCTAGGG1907.1433133E-626.7162672
ATACTCC2756.7484507E-926.36934
TCTAAGG2901.1878001E-825.0053732
GTGCTAT1751.2534362E-424.8637521
AATAGAC1801.5180412E-424.171863
ATAATAC3052.0299922E-823.7755993
TCTATAC3052.0299922E-823.7755993
CTAGGGA3402.4974725E-923.4609223
TTAAGAA4950.023.439384
Produced by FastQC (version 0.11.2)