FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988262
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC46690.23482820674538868No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA45860.23065370660405923No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC42010.21129006137018158No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC41580.2091273685258784No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA39500.1986659705813419No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT38950.1958997355479308No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG35780.1799561627189978No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT32640.16416347543734175No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT27230.13695378174506176No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA27010.13584728773169732No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC26180.13167278759036788No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT24720.12432969095622208No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT23340.11738895578148152No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG22430.1128120941807468No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATC20950.10536840718174968No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA20820.10471456981021615No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA20380.1025015817834873No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA19980.10048977448646104No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT19890.10003711784463012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG18450.043.222249
GTCGCGC18600.042.8736728
TATAGGT18700.042.6562043
GGTCGCG20300.039.2832687
AGGTCGC20800.038.3495676
ATACCCG20450.036.8783235
TCCCCCC9750.035.6900868
ACCTATA21050.035.137291
TACCCGG22050.033.215746
CCTATAA22450.032.30011
CTTCCGA14700.032.063891
CCCCCCG10600.031.4603029
ACCATAC13700.029.1113471
GGGCGAC4750.028.9981947
CTATAAG23750.028.6994482
CCATACT13300.028.3505152
CCGATCT17650.027.9384
ATACTCC13900.027.6498134
TAAGGTG13650.027.624975
CTCCCCC13050.027.2205267
Produced by FastQC (version 0.11.2)