FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5h10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5h10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84395
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1650.19550921263108No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1060.12559985781148172No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG960.1137508146217193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATT100.007079439145.04
TTGATAG100.007079439145.05
TAGTTAT100.007079439145.07
CTTATAT100.007079439145.01
AGTTATA100.007079439145.08
TATAGGT203.853662E-4108.753
CATATCA300.00192958272.58
ATTAAAG300.00192958272.53
GCATATC300.00192958272.57
TGGACAG702.3087705E-762.1428575
TTATCTC350.003555195462.1428574
TATAGTT350.003555195462.1428575
TAAGCAT350.003555195462.1428574
ACGTTAG501.999662E-458.01
AGGGAGT651.08503555E-555.7692346
ATCTCTC400.00603177254.3757
TATCTCA400.00603177254.3755
AGCATAT400.00603177254.3756
TTAAGCA400.00603177254.3753
TATTAAA400.00603177254.3752
Produced by FastQC (version 0.11.2)