FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827140
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA14070.17010421452232996No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13400.16200401383079044No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC12910.156079986459366No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12470.15076045167686244No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12290.14858427835674734No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11180.13516454288270424No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10750.12996590661798485No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10220.12355828517542375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC6300.044.8754548
TCGCGCG6450.042.7079439
TATAGGT7150.040.566733
GGTCGCG7550.037.445747
AGGTCGC7250.037.006656
ACCTATA6200.036.2565161
ATACCCG6350.031.974255
TCCCCCC4100.031.8253678
CCGATCT9300.031.1883984
TACCCGG6550.030.9979366
CTTCCGA8950.030.7876551
TAGGTCG10100.028.718035
CCCCCCG4650.028.0610779
GTCCTAA6250.027.8450051
ATAGGTC11000.027.6867944
TAAGGTG6100.027.3409795
GTATATA3555.456968E-1226.5540681
TTCCGAT10650.026.5540662
CTCCCCC5000.026.0968027
GGGCGAC2259.1140646E-725.7746187
Produced by FastQC (version 0.11.2)