FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396346
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10300.25987394851972767No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9700.2447356602564426No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8990.22682201914488853No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8940.22556049512294815No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8460.21344986451232004No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7830.19755466183587067No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG7690.19402239457443748No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7320.18468711681207833No Hit
GCCTAGGCGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGC7020.1771179726804358No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5790.14608448174070132No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5450.1375061183915064No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5090.12842314543353534No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT4990.1259000973896545No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4750.11984478208434046No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4720.1190878676711762No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG4710.1188355628667881No Hit
CGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCA4690.11833095325801193No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4490.11328485717025023No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC4380.11050950432198131No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4160.10495879862544343No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4080.10294036019033875No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG3990.10066961695084599No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAGT750.001480150338.67811145
TTCCGAT3450.037.822962
TAGATAT800.002034699336.251584
CCGATCT3500.035.215824
TAAGGTG4000.032.6264155
TAGGCGG3500.031.072784
TATAGGT3300.030.7589153
GGCGTAC950.004722883530.5276438
CATAAGG4550.030.2760433
AGGTGCC4150.029.7000877
TATAAGC6150.029.4728263
CACCTAT1753.7657774E-629.0012637
CTAACGT2752.1282176E-1029.001263
CTTCCGA4600.028.3672181
AGGTCGC4200.027.6202496
GCCTATA1050.00770603627.6167661
CTAGGCT3750.027.0678444
CTATAAG5650.026.9446752
TCATAAG5150.026.7453612
TCCCCCC3005.838956E-1026.584498
Produced by FastQC (version 0.11.2)