FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514169
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6130.11922150110177782No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5690.11066400346967631No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT657.3223223E-444.6288834
TAGGATA1105.2483883E-639.5574234
ATGTAAG750.001481220438.6746031
TACAGTG1001.3904214E-436.250397
ACCTATA4600.034.681031
GTAGGAT1301.6498036E-533.471663
GTGCTAA1102.4315783E-432.961311
TACACGG900.00362123432.231975
CCCTAAC3200.031.7252621
ATACTCC1402.7384347E-531.080834
ACTGTAT1402.743901E-531.0717628
GTGTAGG2351.2569217E-930.8573991
TATAGCG950.004718193330.5355535
ATCTTAC950.00472043930.5325831
CTAACGT3151.8189894E-1229.929693
ACCATAC1703.0031551E-629.859071
TACACTG1504.3855423E-529.0087745
TCTAAGG1504.3884538E-529.0059532
TACCCGG4900.028.120756
TGTAGGA1555.4876167E-528.0702762
Produced by FastQC (version 0.11.2)