H3VL5AFXY_n01_p5g6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3VL5AFXY_n01_p5g6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74542
Sequences flagged as poor quality	0
Sequence length	151
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG	317	0.4252636097770384	No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC	286	0.38367631670735963	No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG	233	0.3125754608140377	No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC	185	0.24818223283518018	No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC	145	0.19452120951946553	No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC	130	0.17439832577607256	No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA	126	0.16903222344450108	No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT	115	0.15427544203267957	No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA	111	0.14890933970110812	No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA	106	0.1422017117866438	No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT	101	0.13549408387217945	No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT	101	0.13549408387217945	No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG	98	0.13146950712350083	No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA	96	0.12878645595771512	No Hit
CGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCG	95	0.12744493037482224	No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT	95	0.12744493037482224	No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT	94	0.12610340479192939	No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATA	94	0.12610340479192939	No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT	93	0.12476187920903652	No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG	91	0.12207882804325079	No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA	90	0.12073730246035792	No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC	87	0.11671272571167932	No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT	86	0.11537120012878646	No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT	85	0.11402967454589359	No Hit
CATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTG	84	0.11268814896300072	No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA	82	0.11000509779721498	No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG	80	0.10732204663142927	No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATC	79	0.10598052104853639	No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG	78	0.10463899546564352	No Hit
GCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTG	78	0.10463899546564352	No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG	77	0.10329746988275065	No Hit
CTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCC	76	0.10195594429985778	No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA	76	0.10195594429985778	No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA	75	0.10061441871696494	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCA	10	0.007082395	144.96846	4
TGCGTTG	10	0.007082395	144.96846	5
CAGCGTA	20	3.8552092E-4	108.72634	4
CCCTACC	35	3.4200835E-5	82.83912	1
TTAGAGT	30	0.0019303587	72.48423	4
GGATAAG	35	0.003556629	62.129337	1
TTAGGAC	40	0.006034206	54.36317	3
CCAATCG	40	0.006034206	54.36317	9
TCTAATC	40	0.006034206	54.36317	3
TACCTCC	55	3.2031102E-4	52.7158	4
TAACGTT	145	7.2759576E-12	49.989124	4
CTAACGT	145	7.2759576E-12	49.989124	3
ACTCTCT	45	0.009612683	48.32282	8
CAGGGCA	45	0.009612683	48.32282	4
GATCTAC	45	0.009612683	48.32282	1
CCTATTT	60	4.920748E-4	48.32282	8
AGGGCAT	45	0.009612683	48.32282	5
AACGTTG	155	1.4551915E-11	46.764015	5
GTTGCCG	160	2.0008883E-11	45.302643	8
CCCTAAC	165	2.7284841E-11	43.929836	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content