FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680150
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC31770.4671028449606704No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG29960.44049106814673233No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA27200.39991178416525763No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA25360.37285892817760785No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC24690.36300815996471364No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT22940.3372785414981989No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC21380.3143424244651915No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT20650.3036094979048739No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT18070.2656766889656693No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG16450.24185841358523855No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA14660.2155406895537749No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT14610.21480555759758874No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG13770.20245534073366167No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13210.19422186282437698No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT13090.19245754612953025No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT12630.18569433213261782No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC12610.18540027935014336No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA12360.18172461956921268No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGA12320.18113651400426378No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC12120.17819598617951923No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG12000.1764316694846725No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG11950.17569653752848635No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT11800.17349114165992796No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA11500.16908034992281112No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCG11090.16305226788208485No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC11040.1623171359258987No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG10920.16055281923105197No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA10810.15893552892744248No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG10600.15584797471146072No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA10560.1552598691465118No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG10330.15187826214805558No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT10170.14952583988825993No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA10160.1493788134970227No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG10030.14746747041093877No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG9970.1465853120635154No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATC9910.14570315371609205No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC9860.14496802175990592No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT9850.14482099536866866No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA9750.1433507314562964No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC9750.1433507314562964No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAA9610.1412923619789752No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG9300.1367345438506212No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG9230.1357053591119606No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA9090.13364698963463942No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT9050.13305888406969052No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA8930.1312945673748438No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCG8750.1286480923325737No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG8750.1286480923325737No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAA8670.12747188120267589No Hit
GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC8650.12717782842020142No Hit
AGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGAT8610.12658972285525252No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC8560.12585459089906637No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT8520.12526648533411747No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA8510.12511945894288024No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT8480.12467837976916857No Hit
GCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATA8440.12409027420421967No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA8420.12379622142174519No Hit
CTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGC8300.12203190472689848No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTG8250.12129677277071234No Hit
GCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCC8210.12070866720576344No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGC8080.11879732411967948No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT8040.11820921855473056No Hit
CCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCT8030.11806219216349335No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT7980.11732706020730721No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT7960.11703300742483276No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT7940.11673895464235831No Hit
GCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGA7880.11585679629493495No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT7570.11129897816658091No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC7410.10894655590678527No Hit
CCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT7370.10835845034183637No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG7300.10732926560317578No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA7260.10674116003822685No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC7170.10541792251709181No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG7150.10512386973461736No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT7050.1036536058222451No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT7030.10335955303977064No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT7000.10291847386605896No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTC6890.10130118356244946No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA6870.101007130779975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTC300.001935054372.5039755
TATAGGT11100.051.6019253
TCGCGCG13050.043.884849
AGGTCGC13300.043.066276
GGTCGCG13700.042.331867
GTGTACA700.001056499941.4277951
GTCGCGC14000.040.906948
CATACTC5500.040.8658753
CTATAAG13650.040.3655432
CCTATAA13550.040.1283951
TAAGCAA14050.039.2192275
ATAAGCA13950.038.9806334
CCATACT5600.038.838562
TATAAGC14200.038.804943
GTATTAA750.001483188838.6659431
ACCATAC5450.038.577261
ATACCCG15150.038.2859275
CGAGACA2300.037.83372145
GCAACAT14800.037.7161188
ATACTCC5600.037.54674
Produced by FastQC (version 0.11.2)