FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462416
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10890.2355022317566866No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT9660.20890280613127576No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9430.2039289297948168No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9370.20263139683747966No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9080.1963599875436836No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8220.17776201515518494No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC8050.1740856717760631No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG7220.156136465866233No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT6120.13234836164838587No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5400.11677796616034047No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5270.11396664475277671No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5240.11331787827410816No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC5150.11137157883810249No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4960.10726272447320162No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4960.10726272447320162No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4840.10466765855852739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC1453.8380676E-1044.996587
TCCCCCC2700.042.9596948
CCCCCCG2600.041.8237429
TCGCGCG3550.040.8419659
GTCGCGC3750.038.6637238
TATAGGT3850.037.6594733
GGTCGCG4050.035.7997447
ACCTATA3650.035.7507061
ATACCCG4100.035.3631635
CCTATAA6300.034.5211831
CTAGGGG850.002741490334.1150553
CTATAAG6600.034.050442
CTCCCCC3450.033.6206327
TATAAGC7150.033.4589923
TACCCGG4350.033.33086
CGATCTG3300.032.952045
AGGTCGC4450.032.581796
CTTCCGA4900.032.5485421
TAGACTA900.003627580432.2197724
CCGATCT4900.031.0690634
Produced by FastQC (version 0.11.2)