FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611438
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAGACAGTTCTCAGATCGTTACGCCATTCGTGCAGGTCGGAACTTA10080.16485727089255167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGG553.2156182E-452.7352145
ACTATAT450.00963162648.3406143
TAAGACA4800.045.3193244
GACAGTT4350.043.332777
TCTAGGG700.00105596141.431422
AGACAGT5200.040.438786
GTCTAGG750.001482432938.669331
CTTAAGA4700.038.5664832
AAGACAG5900.038.0989575
GATCTAG1553.4619006E-837.424996
TCTTAAG4850.037.3737031
ACAGTTC6000.036.249538
TTAAGAC5700.035.6193963
GTTTATA1051.8521266E-434.5261842
GGTTTAT850.00274023134.1199951
ATCTAGG1102.4340634E-432.9568141
TTCCGAT19350.031.8497372
CTTCCGA19650.031.7324641
GTATACG950.004722392730.5309144
TCCGATC20600.030.271553
Produced by FastQC (version 0.11.2)