FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623668
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC13980.22415772494339936No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12310.19738065765759985No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11680.18727912928032223No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT10840.17381042477728537No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10670.17108461553262314No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10270.1646709467216532No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG9730.15601249382684376No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC8670.13901627147777343No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8160.13083884374378676No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT6520.10454280161881001No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6270.1005342586119538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC5800.052.4911738
TCGCGCG6250.048.7118159
GGTCGCG6400.047.570137
TATAGGT6550.046.4956473
AGGTCGC7150.042.5939146
CCCCCCG3650.033.7614449
TCCCCCC3700.033.305218
CTAACGT3950.033.043023
CTAGGCT3150.032.2271424
TAGGTCG10150.030.7189775
TAGGCTG3400.029.8574985
CCATACT4450.029.3303182
CGCGGGG1000.006065821729.006754145
ATAGGTC10750.029.0044274
CCTACAT3251.8189894E-1229.0044273
TCTAGAC1504.3915097E-529.0044273
GTATAGG10800.028.8701482
ACCTATA6300.028.7742331
ATAATAC2553.0140654E-928.435713
TATACTG2054.0296436E-728.2970055
Produced by FastQC (version 0.11.2)