FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897459
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG29850.33260572349266093No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC27510.30653210898770866No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG25350.2824641571369834No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC14460.1611215665562438No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13960.15555028140561297No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12630.14073066290493494No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11670.13003379541572374No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11600.1292538154946354No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10840.12078546206567653No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA10100.11253996004274289No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA9940.11075714879454103No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT9810.109308614655377No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT9740.10852863473428871No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT9450.10529728934692281No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA9410.10485158653487235No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA9340.10407160661378403No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC9130.10173166685051907No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG9090.10128596403846861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAA901.3206936E-648.347811
CTAACGT12300.038.911923
TATAGGT11400.038.1671943
TACACTG1157.1299582E-637.8353045
CCCTAAC12600.037.411991
GTACACT800.002034069736.2588354
TATACTG1406.596001E-736.2588355
TAACGTT13300.035.4409644
ACCTATA17000.034.981061
TAATACC1051.8509131E-434.5322234
TACTGTA850.002740059534.122158145
AACGTTG14400.033.740865
ATACCCG17100.033.502315
CCTAACG15600.033.0048332
TACACTC1102.4324695E-432.9625745
TAACACC1551.462E-632.7499124
TACCCGG18500.032.1447566
ATATACC1402.7417953E-531.0790024
ACACATA4200.031.075533145
TGTAGGG2202.2582753E-829.6663192
Produced by FastQC (version 0.11.2)