FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5g10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5g10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687874
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG32990.4795936465108436No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG32460.4718887470670501No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC21860.31779075819118036No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC19380.281737643812675No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA11870.17256067244873333No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC11660.16950778776345668No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11140.16194826378086685No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT11020.16020375824642305No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT10530.15308036064744415No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA10270.14930059865614922No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG10190.14813759496652001No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT9600.13956044275550464No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC9190.13360004884615495No Hit
CGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCG8860.12880265862643447No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8850.12865728316523084No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT8510.12371451748430672No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8500.12356914202310307No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA8300.12066163279903006No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATA8260.12008013095421545No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT7850.11411973704486576No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA7670.11150297874320007No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7650.11121222782079275No Hit
CTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCG7540.10961309774755261No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA7260.10554258483385039No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT7210.10481570752783215No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC7190.10452495660542482No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG7120.10350732837699927No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG7090.10307120199338832No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATC6880.10001831730811167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAA1601.0059011E-940.7948151
TATAGGT10950.037.0899543
TTCCGAT18900.036.453922
CTTCCGA20050.035.0864751
ACCTATA15950.034.5569421
CTAACGT18400.033.8971373
CCCTAAC18600.033.5326541
TAACGTT19200.033.2402154
GTATAGA1551.4603283E-632.752821
ATACCCG17550.032.646185
CCGATCT22800.031.490734
ATAATAC1851.6375634E-731.3617763
ATACTAT1652.3788198E-630.7678056
GCTATAC950.004718600330.5364671
CCTAACG21150.030.518422
AACGTTG20550.029.9978055
GTGTAGA1952.597717E-729.753481
TACCCGG18900.029.1631346
TCCGATC23950.029.0702063
CTCATAC1000.006063299729.0096442
Produced by FastQC (version 0.11.2)