FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648572
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11960.18440512387213756No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11100.1711452236605959No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT10170.1568060292457892No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10080.15541836527016276No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9480.14616727209931973No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8910.13737873358701885No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8550.13182807768451305No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA7060.10885452964358622No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6650.10253294931017681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC3650.041.707588
TCGCGCG3700.041.1439679
TATAGGT4150.036.6854023
GGTCGCG4150.036.682577
CTTCCGA7750.036.4825361
TCTAGGG1406.606733E-736.248672
CCTATAA6400.035.11591
TTCCGAT8200.033.596332
AGGTCGC4550.033.4603086
CCCCCCG2206.220944E-1032.9507989
CCGATCT8450.031.7443984
CTATAAG7350.031.5634672
TACACAG3050.030.9005075
GTCTAGG1204.0573606E-430.2072261
TGCCTAC2451.9699655E-929.5907521
GTGTAGG2701.72804E-1029.5359541
TAGACTA1000.00607399228.9989384
GTCTAAG2003.2524076E-728.9989381
TATAAGC7700.028.2457183
ATAATAC2354.2942702E-827.7649383
Produced by FastQC (version 0.11.2)