FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361917
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG10480.2895691553588253No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8600.2376235435196468No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8360.2309921888167729No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC6940.19175667349143588No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5570.15390269039586424No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC5210.14395565834155344No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT4820.13317970694938344No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4440.12268006200316647No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA4250.11743023953005799No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4110.1135619492867149No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4050.11190411061099645No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA3970.10969365904337182No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG3840.10610167524598181No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.10195707855668563No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA3630.10029923988096717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGT400.006032081354.4107256
CAGACGT1152.4629117E-950.467924
TTAGAGT1751.8189894E-1245.601374
ACACATA2300.040.988182145
TAGACGT1105.2313244E-639.5714384
ATACCCG5000.039.1757245
GACCGTT1701.8226274E-938.381047
TAGAGTG1901.2732926E-1038.1829645
TACCCGG5150.038.0346836
AGGACCG2300.037.850945
GGACCGT2052.8921932E-1035.389096
ACCTATA5150.035.212431
ACCGTTA1656.000482E-835.150318
ACGAGTC2103.7289283E-1034.5464945
TTAGGAC1905.3714757E-934.3646663
TAGGACC1905.3714757E-934.3646664
TGTAGAC850.00273272634.135342
TAACATC3850.033.9183734
CTTCCGA11550.033.9136851
TACGAGT2154.802132E-1033.7430884
Produced by FastQC (version 0.11.2)